BioRT Real Time RT-PCR Kit（SYBR Green）

This product is a 2 × Master Mix for “One-step Real-time PCR” using a thermostable DNA polymerase. This system allows for “One-step Real-time PCR”, including reverse transcription and PCR steps. This reagent is applicable for SYBR Green assay.

This product adopts one tube system, and this system can reduce the potential for contaminating the samples and provide specific, sensitive and convenient analysis of RNA.

Features

Choose F1(SYBR Green I) channels when collecting fluorescent signals and set fluorescent signals detecting at 72 ℃ . Before running Line-Gene Series Real-time PCR detection system, set fluorescent signals detecting at 72℃ for 5 seconds, and then adjust gain to background between 5-15.

Note：Annealing temperature can adjust by primer annealing temperature.

Usage Tips

  1. Total RNA or mRNA can be used as RNA template，and we suggest using high quality RNA;
  2. Quality of primers affect the performance of BioRT Real Time RT-PCR Kit (SYBR Green); Factors such as GC percentage, length of primers and site of primers should be considered and we suggest primers be designed using special software.3.
  3. RNase contamination should be avoided and follow the measures:
  • Ware one time gloves and respirator because of the RNases in saliva and skin;
  • Use special instruments and consumables and handle in specific areas;
  • Consumables should be treated at 180℃ for 2 hours or 37℃ for 12 hours with 0.1% DEPC H2O followed by sterilized at 121℃ for 30min;
4. RT-PCR Mix (SYBR Green) should be slowly pipetted after centrifuging, and put back at -20℃ later.

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